_t content of source leaves of potato plants acclimatised for 14 days in cabinets with artificial diurnal dark/light cycles. The cabinets were off-phased so that plants at different stages in their lighting regime were available at any given time. The AsA_t content of source leaves was monitored for dos4 h in plants sampled simultaneously from the two cabinets. At the end of the dark phase, leaf AsA_t content was approximately 12 mg/100 gFW and it progressively increased following artificial sunrise. After 10 h of light, the leaf AsA_t content increased to approximately 30 mg/100 gFW after which it leveled off. Following artificial sunset the AsA_t level gradually fell back wskazówki dotyczÄ…ce sympatia to approximately 12 mg/100 gFW within 6–10 h.

Changes in AsA_t content of potato leaves as a function of light. Environment chambers were on a 10 h dark – 14 h light cycle as indicated by the brown and yellow panels respectively. At the times shown, source leaves (leaves on the lower four nodes of each stem) were removed from each of three plants, ground in liquid nitrogen and extracted in 5% MPA, 5 mM TCEP (9:1 v/w) prior to quantification by HPLC. Values are represented as mean ± SE, n = 3.

Glasshouse person herbs was in fact gone to live in away from-phased regulated ecosystem compartments (panels Good and you may B) 2 weeks prior to the beginning of the experiment

Table ? Table1 1 shows the AsA_t content of source leaves and tuberising stolons collected at h from the light-phase or dark-phase plants (after collection of phloem exudate for 90 min in a prehumidified atmosphere in leaves). In the table are also reported the chromatographic AsA_t peak areas from phloem exudates collected from source leaves and tuberising stolons of the same plants. (more…)